![]() As a complex polygenic chronic hereditary disease formed by the alternation of environmental and genetic factors, the pathogenesis of essential hypertension is relatively complex. Essential hypertension is one of the leading causes of chronic renal failure and an independent risk factor for highly fatal cardiovascular diseases such as stroke, myocardial infarction, heart failure, and aneurysm ( 2). In the next 5–10 years, the average number of people with hypertension is predicted to rise to 1.1 billion globally ( 1). According to the Fifth National Hypertension Control Survey released by the National Center for Cardiovascular Diseases in 2018, the prevalence of essential hypertension in China has risen to 23.2%. Essential hypertension accounts for 95% of all hypertension cases. It is the most common chronic cardiovascular disease in China. Hypertension, a syndrome characterized by the increasing diastolic and systolic blood pressures of the systemic circulation, is accompanied by various cardio-cerebrovascular diseases. Thus, it may act as a novel screening marker or therapeutic target for essential hypertension in this population. lncRNA PVT1-miR-139-5p-DCBLD2 was indicated to comprise a potential ceRNA regulatory mechanism involved in the development of essential hypertension in the Xinjiang Kazakh population. In 293T cells, lncRNA PVT1 overexpression inhibited miR-139-5p and DCBLD2 levels.Ĭonclusions: Our findings indicate that differentially expressed lncRNAs may be involved in the development of essential hypertension. By constructing the ceRNA regulatory network, we found that lncRNA PVT1-miR-139-5p-DCBLD2 has a potential ceRNA regulatory mechanism involved in the development of essential hypertension in Xinjiang Kazakh people. The differentially expressed mRNAs were found to be primarily involved in the adhesion spot, leukocyte migration via endothelial cells, gap junction, actin cytoskeleton regulation, and extracellular matrix-receptor interaction signaling pathways. The trend of real-time PCR results was consistent with that of the microarray results. Results: In the test group, 396 and 511 differentially expressed lncRNAs and mRNAs, respectively, were screened out. The expressions of miR-139-5p and DCBLD2 after PVT1 overexpression in 293T cells were detected by qRT-PCR and Western blot. The ceRNA regulatory network of lncRNA-miRNA-mRNA was constructed, followed by visualization of the results. GO functional clustering and KEGG pathway analyses were performed for differentially expressed genes. Six differentially expressed lncRNAs were randomly selected for real-time PCR to verify the accuracy and reliability of the gene chip results. After detecting the expression levels of lncRNA and mRNA in the peripheral blood lymphocytes using gene chip technology, their levels in the hypertensive group were compared with those in the control group. Methods: From April 2016 to May 2019, six Kazakh patients with essential hypertension and six Kazakh healthy participants were randomly selected from the inpatient and outpatient cardiology departments of the First Affiliated Hospital of Shihezi University Medical College, Xinjiang. Objective: Here, we aimed to investigate long non-coding RNA (lncRNA) expression characteristics in the peripheral blood lymphocytes of Xinjiang Kazakh people with essential hypertension and the underlying regulatory mechanisms of competing endogenous RNAs (ceRNA). 4Department of Pathophysiology, Shihezi University School of Medicine, Shihezi, China.3Department of Physiology, Shihezi University School of Medicine, Shihezi, China.2NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, China.1Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Ministry of Education, Shihezi University School of Medicine, Shihezi, China. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |